Journal: Biochemical and Biophysical Research Communications

Article Title: p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts

doi: 10.1016/j.bbrc.2012.11.071

Figure Lengend Snippet: p38 MAPK subtype expression in human CF. (A) Relative mRNA levels for p38 subtypes in CF from 8 different patients quantified using real-time RT-PCR. Data are expressed as percentage of GAPDH mRNA levels (2 −ΔCT × 100) and depicted as a box and whisker plot (box defines 25th–75th interquartile range, whiskers depict full range, and horizontal line is median). (B) Whole cell homogenates of CF (5 patients) or human saphenous vein endothelial cells (2 patients) were immunoblotted for expression of p38 subtypes. Blot reprobed with β-actin antibody to confirm equal loading.

Article Snippet: Data are presented as percentage of GAPDH endogenous control mRNA expression (Hs99999905_m1 primers) using the formula 2 −ΔCT × 100.

Techniques: Expressing, Quantitative RT-PCR, Whisker Assay

Journal: Biochemical and Biophysical Research Communications

Article Title: p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts

doi: 10.1016/j.bbrc.2012.11.071

Figure Lengend Snippet: Effect of p38α gene silencing on p38 MAPK pathway signaling. Cells were mock-transfected or transfected with p38α siRNA before incubation for 4 days. (A) mRNA expression of p38 subtypes measured by RT-PCR. Data expressed as percentage GAPDH mRNA levels. ∗∗∗ P < 0.001, NS = not significant ( n = 5). (B) Protein expression of p38 subtypes measured in parallel cultures by immunoblotting. β-Actin blot confirmed equal protein loading. (C) Mock- or p38α siRNA-transfected CF stimulated with 10 ng/ml TNFα or IL-1α for 20 min. P38 MAPK, MAPKAPK2 and HSP27 phosphorylation and expression determined by immunoblotting. Blots reprobed with β-actin antibody to show equal loading.

Article Snippet: Data are presented as percentage of GAPDH endogenous control mRNA expression (Hs99999905_m1 primers) using the formula 2 −ΔCT × 100.

Techniques: Transfection, Incubation, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Phospho-proteomics

Journal: Biochemical and Biophysical Research Communications

Article Title: p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts

doi: 10.1016/j.bbrc.2012.11.071

Figure Lengend Snippet: Effect of p38α gene silencing on IL-6 and MMP-3 expression. CF were mock-transfected (open bars) or transfected with p38α siRNA (filled bars), cultured for 4 days to promote p38α protein silencing, then stimulated with 10 ng/ml IL-1α for 6–24 h. (A) Real-time RT-PCR data for mRNA levels of IL-6, MMP-3 and p38α, expressed as percentage GAPDH mRNA levels. ∗∗∗ P < 0.001, ∗ P < 0.05 for effect of gene silencing (6 h, n = 6). (B) ELISA analysis of IL-6 (6 h, n = 5) and MMP-3 (24 h, n = 4) protein levels in conditioned media from IL-1α treated cells. ∗∗ P < 0.01 for effect of gene silencing.

Article Snippet: Data are presented as percentage of GAPDH endogenous control mRNA expression (Hs99999905_m1 primers) using the formula 2 −ΔCT × 100.

Techniques: Expressing, Transfection, Cell Culture, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Heliyon

Article Title: An increase in urinary primaquine and a reduction in urinary primaquine-5,6-orthoquinone in the Thai population with CYP2D6 reduced enzyme function

doi: 10.1016/j.heliyon.2024.e24351

Figure Lengend Snippet: Baseline characteristics of the participants in the pharmacokinetic study (n = 44) according to the predicted CYP2D6 phenotypes and CYP2D6 activity scores.

Article Snippet: Two different pairs of primers (Hs00010001_cn and Human RNase P) were used to identify the presence or deletion of the entire CYP2D6 gene versus baseline.

Techniques: Activity Assay

Journal: Heliyon

Article Title: An increase in urinary primaquine and a reduction in urinary primaquine-5,6-orthoquinone in the Thai population with CYP2D6 reduced enzyme function

doi: 10.1016/j.heliyon.2024.e24351

Figure Lengend Snippet: Frequency of the CYP2D6 alleles, genotypes, phenotypes and activity scores.

Article Snippet: Two different pairs of primers (Hs00010001_cn and Human RNase P) were used to identify the presence or deletion of the entire CYP2D6 gene versus baseline.

Techniques: Activity Assay

Journal: Heliyon

Article Title: An increase in urinary primaquine and a reduction in urinary primaquine-5,6-orthoquinone in the Thai population with CYP2D6 reduced enzyme function

doi: 10.1016/j.heliyon.2024.e24351

Figure Lengend Snippet: Pharmacokinetic parameters of primaquine and 5,6-orthoquinone primaquine in plasma and urine according to the predicted CYP2D6 phenotypes.

Article Snippet: Two different pairs of primers (Hs00010001_cn and Human RNase P) were used to identify the presence or deletion of the entire CYP2D6 gene versus baseline.

Techniques: Clinical Proteomics

Journal: Heliyon

Article Title: An increase in urinary primaquine and a reduction in urinary primaquine-5,6-orthoquinone in the Thai population with CYP2D6 reduced enzyme function

doi: 10.1016/j.heliyon.2024.e24351

Figure Lengend Snippet: Plasma primaquine concentration time profiles according to the predicted CYP2D6 phenotypes (A) and the CYP2D6 activity scores (B) Data are presented in median ± median absolute deviation (MAD). MAD was calculated as median ( | X i - X ‾ | ) where X ‾ = median ( X i ). AS: activity score; IM: intermediate metabolizers; NM: normal metabolizers.

Article Snippet: Two different pairs of primers (Hs00010001_cn and Human RNase P) were used to identify the presence or deletion of the entire CYP2D6 gene versus baseline.

Techniques: Clinical Proteomics, Concentration Assay, Activity Assay

Journal: Heliyon

Article Title: An increase in urinary primaquine and a reduction in urinary primaquine-5,6-orthoquinone in the Thai population with CYP2D6 reduced enzyme function

doi: 10.1016/j.heliyon.2024.e24351

Figure Lengend Snippet: The cumulative amount-time profile of primaquine (A) and primaquine 5,6-orthoquinone (B) in urine according to the predicted CYP2D6 phenotypes.

Article Snippet: Two different pairs of primers (Hs00010001_cn and Human RNase P) were used to identify the presence or deletion of the entire CYP2D6 gene versus baseline.

Techniques:

Journal: Heliyon

Article Title: An increase in urinary primaquine and a reduction in urinary primaquine-5,6-orthoquinone in the Thai population with CYP2D6 reduced enzyme function

doi: 10.1016/j.heliyon.2024.e24351

Figure Lengend Snippet: The cumulative amount excreted (CAE) in urine of primaquine (A) and primaquine-5,6-orthoquinone (B) and CAE ratio (C) according to the predicted CYP2D6 phenotypes Data are presented as median. p value for the Mann-Whitney U test between normal metabolizers and intermediate metabolizers. CAE: cumulative amount excreted (CAE) in urine; POQ: primaquine-5,6-orthoquinone.

Article Snippet: Two different pairs of primers (Hs00010001_cn and Human RNase P) were used to identify the presence or deletion of the entire CYP2D6 gene versus baseline.

Techniques: MANN-WHITNEY